Mar 1, 2005
By:David V. McCalley
Formic acid often is used for the analysis of peptides in proteomic studies by HPLC-MS, due to its volatility and reduced signal suppression. However, poorer chromatographic performance can be obtained...
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Sep 1, 2004
By:M. de los Santos, C. Nerin, C. Domeno, R. Batlle
This article describes the separation of individual congeners of commonly used fluorescent whitening agents by reversed-phase high performance liquid chromatography and subsequent time-of-flight mass spectrometry analysis. The authors also compare the...
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Mar 1, 2003
By:Ronald E. Majors
Reversed-phase chromatography is by far the most widely used technique in high performance liquid chromatography (HPLC) (1). It is popular because it is applicable to most nonpolar analytes and to...
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Sep 1, 2002
By:Jeffrey R. Mazzeo, Eric S. Grumbach, Steven Collier
The authors describe a method development approach that incorporates four packing materials with unique selectivities. They found that using different packing materials and mobile-phase pH levels was the most powerful...
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Jun 1, 2002
By:John W. Dolan
Many workers use gradient elution scouting runs to screen the retention characteristics of compounds to be separated by reversed-phase liquid chromatography (LC). For example, they might use a 20-min gradient...
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Mar 1, 2002
By:Jessica Wysocki
The driving force of a liquid chromatography separation is determined by solute partitioning between the mobile and stationary phases. Chromatographers must study a wide range of mobile-phase compositions and stationary-phase...
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