Research Group: Dr Jong Shin Yoo, Mass Spectrometry Analysis Group, Department of Instrument Development, Korea Basic Science Institute,
Daejon.
Research Focus: This research group develops
high-throughput analytical methods using nano-, micro-liquid chromatography (LC) or ultra-high pressure LC (UPLC) with mass
spectrometry (MS) to detect very wide and low concentration of peptides from different proteome samples, including human plasma,
brain, tissue, cell line and model bacteria.
High-throughput liquid chromatography mass spectrometry (LC–MS) for quantitative and qualitative analysis has been developed
to quantify very low concentration of peptides in mass spectrometry (MS) mode and obtain structural information in tandem
mass spectrometry (MS–MS) mode.
Dr Jong Shin Yoo also works on the structural identification of phosphoproteins observed in the signal transduction of proteins
in stem cell proliferation by stable isotope labelling with amino acids in cell culture. The method development for the type
and site location of protein phosphorylation helps to identify and quantify the state of phosphorylation between normal and
cancer cell or tissue. They also have expertise in highly sensitive detection of phosphoproteins and glycoproteins by chemical
labelling methods for quantitative proteomics research with high throughput LC–MS. They applied the method to identify and
quantify a different type of phosphoproteins and glycoproteins from liver and colorectal cancer cases. To separate and detect
complex mixture of normal peptides and phosphopeptides, they are using multidimensional connection of ion exchange column
and nanoLC (nLC) system with the on-line detection by matrix-assisted laser desorption ionization or electrospray ionization
in MS.
The group is equipped with liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC–ESI–QTOF–MS),
electrospray ionization ion-trap/FT ion cyclotron mass spectrometry (LC–ESI–IT–FT–MS), liquid chromatography matrix-assisted
laser desorption ionization time-of-flight mass spectrometry (LC–MALDI–TOF–TOF–MS), UPLC ion exchange chromatograph (IEC)
and nanoHPLC (nHPLC). For the database search of various proteins from different biological origin, they use Mascot and Sequest
database with 32 CPU cluster computing system, also serving as an Asia data center for human brain proteome project of Human
Proteome Organization (HUPO).
E-mail: jongshin@kbsi.re.kr
Research Group: Professor Myeong Hee Moon, Separation Laboratory, Institute for NanoBio molecular assemblies, Department of Chemistry, Yonsei
University, Seoul.
Research Focus: This separation laboratory aims to develop multidimensional separation methods for the separation and characterization of
macromolecules, biological macromolecules, nanoparticles, and cells. The focus is to use flow field-flow fractionation (FFF)
techniques that are coupled with other separation methods or detection methods. The development of frit inlet asymmetrical
flow FFF channel has enabled the separation of particles or proteins without stopping migration flow for the relaxation procedure
that is required in FFF techniques.
The developed technique is being used for the separation and molecular weight (MW) characterization of ultrahigh molecular
weight hyaluronic acid by coupling with multi-angle light scattering. Another interesting area is to use polymeric hollow
fibre as flow field flow fractionation (FFF) channel which can eventually be the disposable channel that can reduce any carry
over problem found in bioanalysis. Approaches to miniaturize flow FFF systems was made to develop microscale FlFFF and microbore
hollow fibre FlFFF system that can be used for the separation of low abundance proteins in proteomics. The microbore hollow
fibre FlFFF can be coupled with capillary isoelectric focusing (CIEF) so that protein mixtures can be separated by the differences
of isoelectric point first in CIEF and followed by the differences of molecular weight using HF FlFFF. This is a non-gel based
two dimensional (2D) protein separation device which can perform similar separation obtained by 2D-PAGE.
This method is now being developed further in conjunction with the interface to electrospray ionization tandem mass spectrometry
(ESI-MS–MS) for proteomic research The lab also developed a dual-trap column for the microscale two dimensional (SCX & RPLC)
separation of peptide mixtures prior to capillary LC column so that the combined LC separation can be used for the proteome
analysis by coupling with ESI-MS–MS at nanoflow regime. An interesting project is focused on developing analytical method
for the simultaneous structural identification and quantitation of phospholipids from biological fluid by using nanoflow LC–ESI-MS–MS.
This involves the collaboration with medical centre in relation to the characterization of the phospholipids from human urine
depending on disease status.
E-mail: mhmoon@yonsei.ac.kr
