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Injection Techniques

Retaining Polar Compounds

Jun 1, 2002
By:John W. Dolan

Many workers use gradient elution scouting runs to screen the retention characteristics of compounds to be separated by reversed-phase liquid chromatography (LC). For example, they might use a 20-min gradient...

Communicating with the Baseline

Mar 1, 2002
By:John W. Dolan

The baselines observed in liquid chromatography (LC) separations vary widely in appearance. They range from smooth, straight traces to backgrounds that are filled with humps and bumps. However, if these...

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