In this month's "Column Watch," Ron Majors answers HPLC column questions posed by readers and others.
This "LC Troubleshooting" installment will take a look at pump design, how check valves work, and what steps can be taken to minimize pump problems.
The baselines observed in liquid chromatography (LC) separations vary widely in appearance. They range from smooth, straight traces to backgrounds that are filled with humps and bumps. However, if these...
