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HPLC Columns — Q's and A's

Mar 1, 2006

In this month's "Column Watch," Ron Majors answers HPLC column questions posed by readers and others.

Multidimensional and Comprehensive Liquid Chromatography

Oct 1, 2005

Ron Majors discusses advances in multidimensional chromatography and comprehensive multidimensional chromatography. In particular, the bright future of LC X LC, a technique currently in its infancy, is discussed.

Highlights of HPLC 2005

Sep 1, 2005

HPLC 2005 was held in Stockhom, Sweden, June 26-30. In this month's installment of "Column Watch," Ron Majors covers this year's hot topics including proteomics, column technology - especially monoliths - microfluidics, and multidimensional and high-throughput separations.

Preparative Chiral SFC as a Green Technology for Rapid Access to Enantiopurity in Pharmaceutical Process Research

Jun 1, 2005

This month's installment of "Column Watch" provides an overview of green chemistry considerations for the kilogram-scale preparative chromatographic separation of enantiomers, focusing especially on recent results from the authors' laboratories, illustrating solvent and time savings realized with preparative supercritical fluid chromatography (SFC). They compare optimized high performance liquid chromatography and SFC in terms of energy efficiency and throughput.

Preparative Chiral SFC as a Green Technology for Rapid Access to Enantiopurity in Pharmaceutical Process Research (PDF)

Jun 1, 2005

Recently, the subject of green chemistry has received considerable attention from academic and industrial researchers alike. While the central tenets of waste reduction, process economy, and elimination of risks and...

The Role of the Column in Preparative HPLC

Sep 1, 2004

In the course of writing this year's Pittcon coverage of high performance liquid chromatography (HPLC) columns (1,2), I observed that large numbers of columns and packing materials designed for preparative...

Are You Getting the Most Out of Your HPLC Column?

Mar 1, 2004

This months Column Watch discusses low-dispersion columns, describes how to modify high performance liquid chromatography systems to work with the small-volume columns, and provides guidance about obtaining the optimum capabilities from these columns...

The Use of Micrometer-Sized Particles in Ultrahigh Pressure Liquid Chromatography

Nov 1, 2003

During the past 20 years, researchers have investigated several approaches for improving the separation power of standard chromatography columns, which provide 20,000 theoretical plates. Packing conventional 3- and 5-µm...

The Cleaning and Regeneration of Reversed-Phase HPLC Columns

Mar 1, 2003

Reversed-phase chromatography is by far the most widely used technique in high performance liquid chromatography (HPLC) (1). It is popular because it is applicable to most nonpolar analytes and to...

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